Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits NF-κB signaling pathway activation and inflammatory factor secretion in SCs. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in SCs after CXCL12 treatment. ( B - C ) Quantification of gray values for the NF-κB, p-NF-κB, κBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in SCs after CXCL12 treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits the inflammatory response in SCs via the NF-κB signaling pathway. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in SCs after NF-κB overexpression. ( B - C ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in SCs after NF-κB overexpression. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing, Over Expression

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits SC ferroptosis, thereby mitigating the inflammatory response. ( A ) Western blot analysis of the expression levels of ferroptosis-related proteins ACSL4, GPX4, and FSP1 in SCs after FAC treatment. ( B - D ) Densitometric analysis of proteins ACSL4, GPX4, and FSP1. ( E , F ) Fluorescence images of ROS and Fe 2+ in SCs after FAC treatment, Scale bar = 50µm. ( G , H ) Quantification of the relative fluorescence intensity of ROS and Fe 2+ . ( I , J ) Expression levels of MDA and GSH in SCs after FAC treatment. ( K ) Western blot analysis of the expression levels of NF-κB, p-NF-κB, IκBα, and p-IκBα in SCs after FAC treatment. ( L , M ) Densitometric analysis of proteins NF-κB, p-NF-κB, IκBα, and p-IκBα. ( N , O ) Expression levels of IL-1β and TNF-α in SCs after FAC treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing, Fluorescence

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits NF-κB signaling pathway activation and inflammatory factor secretion in the sciatic nerve. ( A ) Western blot analysis of NF-κB, p-NF-κB, IκBα, and p-IκBα protein expression levels in the sciatic nerve after CXCL12 treatment. ( B - C ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα, and p-IκBα proteins. ( D - E ) Levels of IL-1β and TNF-α in the sciatic nerve after CXCL12 treatment. The data are presented as the means ± SDs, n=3, **p < 0.01, ***p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Inflammation

Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

doi: 10.1007/s10753-026-02453-2

Figure Lengend Snippet: CXCL12 inhibits sciatic nerve ferroptosis, thereby mitigating the inflammatory response. ( A ) Western blot analysis of ferroptosis-related protein expression levels (ACSL4, GPX4, FSP1) in the sciatic nerve after FAC treatment. ( B - D ) Quantification of the gray values for the ACSL4, GPX4, and FSP1 proteins. ( E ‒ G ) Levels of Fe 2+ , MDA, and GSH in the sciatic nerve after FAC treatment. ( H ) Western blot analysis of NF-κB, p-NF-κB, IκBα and p-IκBα protein expression levels in the sciatic nerve after FAC treatment. ( I , J ) Quantification of the gray values for the NF-κB, p-NF-κB, IκBα and p-IκBα proteins. (K, L) Levels of IL-1β and TNF-α in the sciatic nerve after FAC treatment. The data are presented as the means ± SDs, n=3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: An enzyme-linked immunosorbent assay was used to measure the concentrations of the inflammatory factors IL-1β (Elabscience, E-HSEL-R0002, China) and TNF-α (Elabscience, E-HSEL-R0001, China) in the cell culture supernatant (centrifuged at 3000×g for 15 min).

Techniques: Western Blot, Expressing

Journal: Cell & Bioscience

Article Title: The mechanisms by which hypothalamic neuroinflammation induced by neonatal cerebral ischemia–hypoxia leads to decreased thymic function via the HPA axis

doi: 10.1186/s13578-026-01543-w

Figure Lengend Snippet: HI induced the activations of TLR4/HMGB1/NF-κB signaling and microglia, leading to neuroinflammation in hypothalamus. A – B TNF-α and IL-1β on days 1, 3, 7, 14 and 21 after HI, n = 5–8. C Double immunofluorescence staining of Iba-1 and CD68. Scale bar = 20 μm. D Number of Iba-1 positive cells ( n = 4). E CD68 + / Iba-1 + cells rate ( n = 4). F – J CD11b, HMGB1, TLR4, p-P65-NF-κB and P65-NF-κB protein levels on day 7 after HI, n = 5. Data were expressed as the mean ± SD. Comparisons between the two groups were made using an unpaired T-test, * P < 0.05, ** P < 0.01, *** P < 0.001 versus Sham group.

Article Snippet: Serum hormone levels, along with hypothalamic tissue hormone and cytokine levels, were measured using CRH, ACTH, and CORT ELISA kits (Omnimabs, USA), as well as TNF-α and IL-1β ELISA kits (Proteintech, China), following the manufacturers’ instructions.

Techniques: Double Immunofluorescence Staining

Journal: Neurotoxicity Research

Article Title: Chronic Isolation Stress Affects Subsequent Crowding Stress-Induced Brain Nitric Oxide Synthase (NOS) Isoforms and Hypothalamic-Pituitary-Adrenal (HPA) Axis Responses

doi: 10.1007/s12640-019-00067-1

Figure Lengend Snippet: The effect of isolation stress (IS) for 11 days on plasma ACTH, corticosterone (CORT), and interleukin-1β (IL-1β) levels. Rats exposed to isolation stress were kept 1 per cage for 11 consecutive days and decapitated. Graphs represent the mean ± SEM, n = 10–12 rats per group. Data were analyzed by Student’s t test: ++ p < 0.01 and +++ p < 0.001 vs. non-stressed control group

Article Snippet: Plasma CORT, ACTH, and IL-1β concentrations were determined using the following kits: Rat/Mouse Corticosterone EIA kit (Immunodiagnostic Systems, Boldon, UK), ACTH Rat/Mouse EIA kit (Phoenix Pharmaceuticals, Burlingame, CA, USA) and Rat IL-1β ELISA kit (BioVendor, Brno, Czech Republic) following the manufacturer’s instructions.

Techniques: Isolation

Journal: Neurotoxicity Research

Article Title: Chronic Isolation Stress Affects Subsequent Crowding Stress-Induced Brain Nitric Oxide Synthase (NOS) Isoforms and Hypothalamic-Pituitary-Adrenal (HPA) Axis Responses

doi: 10.1007/s12640-019-00067-1

Figure Lengend Snippet: Comparison of the effect of isolation stress (IS) (for 11 days), crowding stress (CS) for 3 ( a , d , g ), 7 ( b , e , h ), and 14 days ( c , f , i ), and IS + CS (for 3, 7, and 14 days) on IL-1β ( a , b , c ), ACTH ( d , e , f ), and corticosterone levels ( g , h , i ) in plasma. Graphs represent the means ± SEM of 10–12 rats per group. Values are expressed as the mean ± SEM, n = 10–12 and were analyzed by two-way ANOVA and post hoc Tukey’s multiple comparison test: + p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. non stressed control group; *** p < 0.001 vs. IS; ### p < 0.001 vs. CS

Article Snippet: Plasma CORT, ACTH, and IL-1β concentrations were determined using the following kits: Rat/Mouse Corticosterone EIA kit (Immunodiagnostic Systems, Boldon, UK), ACTH Rat/Mouse EIA kit (Phoenix Pharmaceuticals, Burlingame, CA, USA) and Rat IL-1β ELISA kit (BioVendor, Brno, Czech Republic) following the manufacturer’s instructions.

Techniques: Isolation